Proton NMR Analysis of Human Prostate Tissue with Slow Rate High Resolution Magic Angle Spinning: (A+B-|A-B|)
نویسندگان
چکیده
L. L. Cheng, A. G. Zepeda, C. Wu, R. G. Gonzalez, A. Bielecki, D. Cory MGH/Harvard Med School, Boston, MA, United States, MIT, Cambridge, MA, United States Synopsis. Applying high resolution magic angle spinning (HRMAS) NMR spectroscopy to investigate cellular metabolism of diseases has encouraged exploration of slow spinning methodologies to better preserve tissue pathology structures against HRMAS centrifuging damages. Spinning sidebands (SSB) resulting from slow spinning must be eliminated to prevent complications to metabolite spectra. A novel scheme employing two spectra (A, B) of different slow spinning rates is evaluated with human prostate tissue. By editing these spectra according to the formula A+B-|A-B|, SSB free spectra can be obtained for metabolite quantification, resulting in spectra that are comparable to those measured with high rate spinning.
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